Analysis of Protein‐Protein Interactions by Split Luciferase Complementation Assay
نویسندگان
چکیده
منابع مشابه
Application of a split luciferase complementation assay for the detection of viral protein-protein interactions.
Intraviral protein-protein interactions are critical for virus survival in the host. Discovery of such interactions is important to understand molecular mechanisms of viral replication and pathogenesis. The development of a cell-based assay that can be employed to examine systematically viral protein interactions is described. The method, known as the split luciferase complementation assay (SLC...
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Bioluminescent proteins such as luciferases are unique analytical tools with high sensitivity and wide dynamic ranges. However, applicability of the proteins has remained limited to reporter gene analysis. Split luciferase complementation technique is a new powerful strategy to detect protein-protein interactions and their related intracellular signaling in living cells. This review presents a ...
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ژورنال
عنوان ژورنال: Current Protocols in Toxicology
سال: 2019
ISSN: 1934-9254,1934-9262
DOI: 10.1002/cptx.90